The serological pipette is mainly termed to be a laboratory instrument, which can transfer liquids measured in volume by the ml count. Most people have graduations on the side for measuring liquid being aspirated or dispensed. The major step while plating or culturing cells is to get proper distribution throughout the solution. The proper use of the pipettes is gentle and efficient means of mixing cell suspensions.
You can also use the equipment for mixing chemical solutions and reagents. After isolation or treatment of the experimental cell cultures, the pipettes are then quite useful in transferring the cell colonies for further empirical expansion or analysis. You can further use the item for layering reagents carefully for creating density gradients. Some examples are ficolin gradient as used for purifying blood plasma cells.
More about the objects and usability:
You can always procure serological pipette in various sizes and options. There are mainly four varieties available online of the disposable pipette. The calibrated ones will deliver volumes, which can range right from 0.1 ml to the 25 ml count or mark.
- Some of the most common sizes as used over here are 5, 10 and 25 ml. These items are widely used for liquid transfer, which is free from any pathogenic micro-organisms.
- For any kind of microbiology or tissue culture experiments, the reliable centers are offering pre-sterilized transfer pipettes, which can always be heat sealed for storing airtight samples right now.
- Some of the common uses of these items are immunology, urinalysis, and hematology and even in blood banking.
- These items are amazing for applications, which involve tissue cultures. The products comprise of low-affinity surfaces, designed for reducing protein, sample or cell loss.
- The products are available in 10 different designs. There is a high chance that at least one of them might meet requirements for some drops as per the ml, bulb draw, pipette capacity or the length.
- You can often take hold of these items for biosafety level on some of the lab-based experiments. These pipettes are the only type, meant for the BSL 2 organisms, where the Bunsen burners might fail to work. They are often recommended for applications involving fluid agar transfer.
Liquids aspirated from pipettes:
It is vital to use pipettor dispenser for fluid transfer. It can help in negating ancient technique, used by researchers and primarily stated as mouth pipetting. Transferring liquid with the help of this method is not quite recommended. There are some serious side effects, which can result from the fluid entering mouth.
You are asked or advised to use either a bulb or pump for aspiration and dispensing. The least accurate one is the bulb. It can be used for transferring non-specified liquid amounts. The pump is also made more precise. It can allow for some accurate regulations of fluid when dispensing repeat liquid volume solutions now. There are mainly two triggers related to the pump. One is depressed for the aspiration, and the other one is used for dispensing.